A Smartphone App for Individual Xylazine/Ketamine Calculation Decreased Anesthesia-Related Mortality in Mice

Currently, experimental animals are widely used in biological and medical research. However, the scientific community has raised several bioethical concerns, such as number of required to achieve reproducible statistically relevant results. These concerns involve aspects related pain, discomfort, unwanted animal loss. Retrospectively, we compare two different approaches for anesthesia dosage: a mobile app dose calculation standard calculation. A total 939 C57BL/6J Swiss mice were analyzed. We collected data on intraoperative anesthesia-related mortality described electronic or physical handwritten records. Our results showed that approach significantly reduces anesthetic-related deaths upon using doses ketamine xylazine. The suggest can be minimized even more information technology approaches, helping solve an old but transversal challenge researchers working with mice. is free open code which could implemented worldwide essential requirement all anesthetic procedures xylazine combination. As app, Labinsane initiative also represent starting point unify validate other species strains.